Regeneration of tissue to trade damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa current a short lived scaffold to info new tissue progress and or- nization. Typically, they have to be biodegradable, displaying good cell atta- ment and proliferation and they should possess relevant mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements nevertheless lack cell-adhesion peptides on their flooring to strengthen cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a man-made polymer coated with ECM proteins would finish in a scaffold that mimics the pure cellular environment with enhanced cell attachment and p- liferation. The model new bioactive scaffold could be made by combining a man-made polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of determining polymers that give biggest results in phrases of CHO cell attachment and progress. Classical strategies to seek out out biomass are inappropriate to guage three-D buildings. Thus a screening system based mostly totally on safe GFP expressing CHO cells was used to match the completely totally different scaffolds. Straightforward uorescent measurement after cell lysis permits determining cell attachment and p- liferation on synthetic polymers. Lastly CHO cells producing human recombinant collagen I and elastin have been generated. We confirmed that every proteins are expressed and secreted by CHO DG44 cells. 2 Provides and Methods 2.
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