Consists of all the data required to produce monoclonal antibodies inside the laboratory and to put collectively them to be utilized in a multitude of given functions. Manufacturing procedures are dealt with in chronological order, beginning with main tissue custom strategies, immunization strategies and screening examine design, adopted by manufacturing of hybridoma cell strains and first antibody characterization, purification and labeling. Each chapter incorporates explanatory textual content material on each step with comparative analysis of methods the place relevant. All important experimental protocols are launched in a self-contained format that is simple to adjust to inside the laboratory. Numerous protocols are provided the place associated; for others not included in full, provide references are launched. Surveys the current standing of human hybridoma manufacturing and antibody engineering using molecular biology strategies.
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